10/31/2023 0 Comments Jing zhang fbbSequential activation of the AKT pathway in human osteoblasts treated with Oscarvit: a bioactive product with positive effect both on skeletal pain and mineralization in osteoblasts. Gorogh, T., Quabius, E.S., Georgitsis, A., Hoffmann, M., and Lippross, S. The functional role of the JAK-STAT pathway in post-infarction remodeling. 10.1038/nm1075 Suche in Google ScholarĮl-Adawi, H., Deng, L., Tramontano, A., Smith, S., Mascareno, E., Ganguly, K., Castillo, R., and El-Sherif, N. Progenitor cell trafficking is regulated by hypoxic gradients through HIF-1 induction of SDF-1. 10.3181/00379724 Suche in Google ScholarĬeradini, D.J., Kulkarni, A.R., Callaghan, M.J., Tepper, O.M., Bastidas, N., Kleinman, M.E., Capla, J.M., Galiano, R.D., Levine, J.P., and Gurtner, G.C. The identification of JAK2 tyrosine kinase as a signaling molecule for growth hormone. 10.1126/science.1249783 Suche in Google ScholarĬarter-Su, C., Argetsinger, L.S., Campbell, G.S., Wang, X., Ihle, J., and Witthuhn, B. Mechanism of activation of protein kinase JAK2 by the growth hormone receptor. Identification of JAK2 as a growth hormone receptor-associated tyrosine kinase. This suggests the possible role of JAK2/STAT3 signaling in hypoxia-induced osteogenic differentiation of MSCs and bone defect healing.Īrgetsinger, L.S., Campbell, G.S., Yang, X., Witthuhn, B.A., Silvennoinen, O., Ihle, J.N., and Carter-Su, C. Moreover, AG490 inhibited phosphorylation of STAT3, P38, JNK and AKT. Our results clearly showed the inhibitory effect of AG490 on proliferation and osteogenic differentiation of BMSCs, bone regeneration and bone defect healing. Histology and μ-computed tomography (CT) data showed that AG490 treatment inhibits bone regeneration and bone defect healing. Immunohistochemistry showed high numbers of pJAK2, pSTAT3 and ALP positive cells and AG490 reduced this effect in vivo. Inhibition of JAK2 reduced phosphorylation of STAT3, AKT, P38, and JNK phosphorylation. Inhibition of JAK2 reduced ALP activity and matrix mineralization in BMSCs culture. Col1α, Alp and Ocn expression in mRNA and protein levels. AG490 inhibited BMSCs proliferation and osteogenic differentiation markers, i.e. We used AG490 to inhibit the JAK2/STAT3 signaling in a mice bone marrow stromal cells (BMSCs) culture. In this study, we aimed to analyze the effect of AG490, a JAK2-specific inhibitor, on MSCs proliferation and osteogenic differentiation as well as in bone defect healing. However, the role of JAK2/STAT3 in the osteogenic differentiation of MSCs and bone defect healing is still not fully understood. Mesenchymal stem cells (MSCs) undergo osteogenic differentiation during bone defect healing.
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